IKAN TRANSGENIK PDF

IDENTIFIKASI IKAN CUPANG (Betta imbelis) TRANSGENIK FOUNDER MEMBAWA GEN PENYANDI HORMON PERTUMBUHAN; Identification of Transgenic. Ikan transgenik F-0 dibuat dengan menggunakan metode transfeksi. Identifikasi dilakukan menggunakan metode RT-PCR. RNA total. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are.

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Jurnal Akuakultur Indonesia Vol 6, No 1 ilan Tingkat ekspresi transgen yang tinggi pada jaringan ikan F2 yang diperiksa telah diperoleh. Persilangan antara ikan transgenik tersebut dengan ikan normal menunjukkan transmisi transgen ke keturunannya mengikuti hukum segregasi Mendel.

This review describes various techniques of gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture. Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis.

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Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga. This Author published in this journals. Thus, we found that the qTR-PCR to be effective for a rapid and precise determination of zygosity in transgenic fish. This method needs relatively large amounts of fish material and is time- and labor-intensive.

Dengan demikian, sebuah sistem transgenik untuk memodifikasi biosistesa asam lemak pada ikan telah dikembangkan. Data dianalisis menggunakan metode pembandingan nilai cycle threshold.

This technique has been applied to several commercially valuable species. Now, a transgenic system to study the modification of fatty acid biosynthesis pathways in fish was established.

New approaches addressing this problem could be of great help for fish biotechnologists. Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi. Highly unsaturated fatty acids HUFAespecially eicosapentaenoic acid EPA and docosahexaenoic acid DHA have long been recognized for its beneficial effect for human health tfansgenik development.

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JURNAl AQUACOASTMARINE

This technique could be useful in the establishment of breeding programs for mass transgenic fish production and in experiments in which zygosity effect could have a functional impact. The intensity and capacity of production is expected to increase using biotechnology approach. The first F1 and second Transsgenik generation was produced by crossing the transgenic founder F0 and F1, respectively, with wild-type fish.

Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived ikkan mating the transgenic fish and wild-type counterpart. The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis.

Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti bioteknologi perikanan. Transgenic individuals were identified by polymerase chain reaction PCR and their expression levels were analyzed by reverse transcription PCR.

Data were analyzed using the comparative cycle threshold method. Transgen telah ditransmisikan ke ikan generasi F2 dengan mengikuti pola segregasi Mendel. The transmission rate of transgene into F1 generation was 4.

Teknik ini dapat berguna dalam program produksi ikan transgenik secara massal dan dalam percobaan dimana faktor sigositas memberikan pengaruh nyata. Jurnal Akuakultur Indonesia Vol 6, No 2 Mating of those transgrnik with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws.

Jurnal Akuakultur Indonesia Publisher: Jurnal Akuakultur Indonesia Vol 2, No 1 Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population.

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Pada penelitian ini, kami menggunakan metode PCR real-time kuantitatif krt-PCR untuk menganalisa sigositas pada satu strain ikan zebra Danio rerio transgenik yang membawa gen D6-desaturase-like dari ikan salmon masu, Oncorhynchus trajsgenik. Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur.

Expression of transgene in F2 generation was varied between strains regarding as the mosaics of F0 fish. Rapid method for identification of transgenic fish zygosity Jurnal Akuakultur Indonesia Vol 6, No 2 Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia.

In this experiment, we applied a quantitative real-time PCR qr-PCR method to analyze trangenik in a stable line of transgenic zebrafish Danio rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene.

One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique. Salah satu teknik modern yang diduga akan menjadi trangsenik yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Recently, global food security has become a hot issue by the public in national as well as out of the country.